Instrumen Diagnostik
The Principle of Direct Chemiluminescence Immunoassay
October 12 , 2024
      Chemiluminescence Analyzer consists of the two parts of fully automated reaction system and chemiluminescence assay system. Fully automated reaction system is the process of indirect specific binding of luminescent marker to solid phase carrier by means of incubation and magnetic separation. Chemiluminescence assay system refers to the process of photomultiplier’s capture of photon with maximum wavelength of 430nm emitted by luminescent marker upon oxidization with hydrogen peroxide when acid environment suddenly changes into alkaline environment.

      The system uses acridinium ester and its derivatives as luminescent marker, and it becomes luminous through injection of activator within seconds, which makes it flash-type chemiluminescence. As luminescent marker used for assay and analysis, acridinium ester and its derivatives boasts of simple reaction, rapidness, absence of catalyst, excellent stability, less nonspecific binding, high sensitivity and other strengths, which make it a new effective compound.

      The core detector of this instrument is photomultiplier (PMT), which detects single photons and transfer to amplifier. High-voltage current’s added for amplification, amplifier converts analog current into digital current, digital current transfer luminescence signal to mainboard for calculation of relative luminescence unit (RLU), and contents of test antigens or antibodies in measurement sample are calculated through standard curve.

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